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The aim of this study was the evaluation of the influence of temperature, initial organic load, and C/N ratio in the biochemical hydrogen potential (BHP) by dark fermentation (DF) of mixtures composed of residual biomass derived from the Colombian agro-industry (pig manure, cocoa mucilage, and coffee mucilage) to contribute to residue management and the development of a bio-based economy in Colombia. A Box-Behnken experimental design was constructed, where the variables were as follows: three initial organic loads (2, 5, and 8 g COD/l), three C/N ratios (25, 35, and 45), and three different coffee and cocoa initial COD ratios (coffee mucilage (CFM):cocoa mucilage (CCM)) (1:3, 2:2, and 3:1). The experiments were carried out at thermophilic environments of 55 °C and 45 °C. Sludge from an anaerobic digester pretreated by thermal shock was used as an inoculum. The results of the experimental designs …