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The singlet oxygen quantum yield of photodynamic agents was measured at 546nm, 630 nm and on the far-red absorption peak. The technique employed is available in most laboratories, in which the photosensitization of lysozyme is used as an internal actinometer. Measurements in a pH 7.4 phosphate buffer plus 1% Triton X-100 (PB/X100) are scaled to 0.52 for methylene blue in the phosphate buffer. The average quantum yields are: hematoporphyrin IX (0.73), protoporphyrin IX (0.56), zinc protoporphyrin IX (0.91), mesotetra-(4-sulfonato-phenyl)porphine (0.61), Photofrin^R (0.89), benzoporphyrin derivative monoacid ring-A (0.84), chlorin e6 in PB (0.64), pheophorbide a (0.69), and aluminum phthalocyanine tetrasulfonate (0.38). Protection factors were measured for added azide ion, 1,4-diazabicyclo[2,2,2]-octane, and superoxide dismutase. Spectral evidence is presented for chlorin e6 interactions with PB …