Autores
María Isabel Jercic, Graciela Santillan, Susana Elola, William Quispe Paredes, Lidia B Conza Blanco, Noelia Morel, Rodrigo Villegas, Baldomero Molina Flores, Cesar M Gavidia, Marta Cabrera, Alexandre Guerra Dos Santos, Manuel J Sanchez-Vazquez, Melody J Maxwell, Marco A Vigilato, Edmundo Larrieu, Víctor J Del Rio Vilas
Fecha de publicación
2019
Revista
Revista Panamericana de Salud Pública
Volumen
43
Editor
Pan American Health Organization
Descripción
Objective.
To compare the performance of polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) tests for diagnosing Echinococcus granulosus in dog feces among national reference laboratories in Argentina, Chile, Peru, and Uruguay.
Methods.
National laboratories affiliated with the Ministry of Health/Agriculture of each country exchanged panels of 10 positive/negative samples obtained from their regular national surveillance programs in November 2015–November 2016. All laboratories applied PCR; two also applied ELISA techniques. Sensitivity and specificity were determined for each laboratory and concordance of results among the laboratories was evaluated by Cohen Kappa coefficient.
Results.
Poor concordance (3 of 10 paired comparisons had values of Kappa> 0.4), low sensitivity and specificity across all laboratories, and poor performance of both techniques in detecting E …
Artículos de Google Académico
MI Jercic, G Santillan, S Elola, WQ Paredes… - Revista Panamericana de Salud Pública, 2019