Autores
Irina Gheorghe, Ângela Novais, Filipa Grosso, Carla Rodrigues, Mariana Carmen Chifiriuc, Veronica Lazar, Luísa Peixe
Fecha de publicación
2015/4/1
Revista
Journal of Antimicrobial Chemotherapy
Volumen
70
Número
4
Páginas
1016-1020
Editor
Oxford University Press
Descripción
Objectives
The present study was designed to provide a snapshot on carbapenemase-producing Pseudomonas aeruginosa (n = 11) and Acinetobacter baumannii (n = 7) isolates in hospitalized patients (November 2011, January–March 2012) from two main hospitals in Bucharest, south Romania.
Methods
Clonality among isolates was established by PFGE, MLST and Fourier transform infrared spectroscopy. Carbapenemases were screened by the Blue-Carba test, PCR and sequencing. Transferability of blaOXA-23 was tested by conjugation and plasmid typing (number, size and identity) was assessed by S1-PFGE, replicon typing, hybridization and PCR mapping.
Results
All P. aeruginosa isolates carried chromosomally located blaVIM-2, associated with a common class 1 integron (aacA7-blaVIM-2) or an atypical configuration (aacA7-blaVIM-2 …
Artículos de Google Académico
I Gheorghe, Â Novais, F Grosso, C Rodrigues… - Journal of Antimicrobial Chemotherapy, 2015