Autores
Ana Elena Pérez-Cobas, María José Gosalbes, Anette Friedrichs, Henrik Knecht, Alejandro Artacho, Kathleen Eismann, Wolfgang Otto, David Rojo, Rafael Bargiela, Martin von Bergen, Sven C Neulinger, Carolin Däumer, Femke-Anouska Heinsen, Amparo Latorre, Coral Barbas, Jana Seifert, Vitor Martins Dos Santos, Stephan J Ott, Manuel Ferrer, Andrés Moya
Fecha de publicación
2013/11/1
Revista
Gut
Volumen
62
Número
11
Páginas
1591-1601
Editor
BMJ Publishing Group
Descripción
Objective
Antibiotic (AB) usage strongly affects microbial intestinal metabolism and thereby impacts human health. Understanding this process and the underlying mechanisms remains a major research goal. Accordingly, we conducted the first comparative omic investigation of gut microbial communities in faecal samples taken at multiple time points from an individual subjected to β-lactam therapy.
Methods
The total (16S rDNA) and active (16S rRNA) microbiota, metagenome, metatranscriptome (mRNAs), metametabolome (high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight mass spectrometry) and metaproteome (ultra high performing liquid chromatography coupled to an Orbitrap MS2 instrument [UPLC-LTQ Orbitrap-MS/MS]) of a patient undergoing AB therapy for 14 days were evaluated.
Results
Apparently oscillatory population dynamics were observed …
Artículos de Google Académico
AE Pérez-Cobas, MJ Gosalbes, A Friedrichs, H Knecht… - Gut, 2013