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The earlier studies aimed at investigating the response of tissue fatty acyl composition to variation in dietary fat were predominantly concerned with the state of essential fatty acid deficiency [91-95] and its correction. In dietary studies a fat-free diet or a diet containing only saturated fatty acids is often still used as one extreme point of reference. Later, interest developed in the effect of feeding levels of polyunsaturated fatty acids much in excess of the minimum dietary requirement for health, partly due to suggestions that this could have a beneficial prophylactic effect against heart disease [98]. More recently a growing interest in n-3 fatty acids [99], again partly prompted by suggestions that these could be beneficial in protecting against heart disease [100,101], has led to investigations of the response of tissues to the long chain n-3 fatty acids found most plentifully in marine animals. Other work has been directed at …