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Startup ecosystems have a positive impact on the creation of new businesses and the entire economy and accordingly receive attention from both academics and politicians. However, while entrepreneurial ecosystems reflect a high level of entrepreneurial activity, they differ significantly in terms of the proportion of female founders, which means some ecosystems do not fulfill their true potential. This study uses qualitative comparative analysis to explore the combinations of ecosystem characteristics explaining a high proportion of female founders in the 20 most successful startup ecosystems worldwide. The results suggest two different configurations explaining a high proportion of female founders and reveal which issues require attention on a metropolitan level and which issues might require national policy makers to become involved. These findings contribute to the ecosystem literature and link to the stream of …