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ONE of the main obstacles in breeding Citrus arises from the fact that most varieties are apomictic and polyembryonic. This implies that in the nucellus of the mother plant asexual embryos develop together with, or instead of, zygotic embryos. This phenomenon produces a large proportion of nucellar offspring, genetically identical to their female progenitor. It is, therefore, of great interest to the breeder to be able to identify at an early stage of seedling development those zygotic plants that carry a combination of the desirable characters of both progenitors. An early recognition of these seedlings would allow for more accurate and economic methods of selection. At the same time, it would provide information on the frequency and identification of nucellar offspring that may be used to propagate a variety free of most virus infections.

The problem of early recognition of progenies reproduced by apomixis and …